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Bio rad 20 bp ladder.
Standard ez load and amplisize rulers.
Bio rad molecular rulers are dna standards with precisely defined size intervals between bands dna ladders for simplified estimation of the length of nucleic acids separated on agarose gels.
Monitor electrophoresis and blotting experiments estimate molecular weights.
50 bp dna ladder is stable for at least 3 months at 4 c.
For long term storage store at 20 c.
General description sigma s pcr 20 bp low ladder contains 50 dsdna fragments from 50 1 000 bases evenly spaced in 20 bp repeats.
200 μl 0 2 μg μl dna standard 500 8 000 bp 16 bands in 500 bp increments 100 applications.
Gel images were managed using the bionumerics software package version 4 0 applied maths sint.
Compare 20 bp ladders from leading suppliers on biocompare.
Molecular mass rulers bio rad precision molecular mass rulers dna ladders allow accurate dna quantitation in gels making them ideal for densitometry or image analysis.
The size markers used were a 100 bp ladder ez load 100 pb pcr molecular ruler biorad marnes la coquette france or 20 bp ladder ez load 20 pb molecular ruler biorad marnes la coquette france according to the tandem repeat unit length.
Bio rad provides three types of molecular rulers.
1x gel loading dye purple no sds.
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Or 0 5 2 5 µl for silver staining up to 1 000 applications.
Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
Dna may be denatured if diluted in dh 2 o.
Standard ez load and amplisize rulers.
Bio rad provides three types of molecular rulers.
We recommend loading 0 5 1 0 µg of 50 bp dna ladder diluted in sample buffer.
Use 1 0 5 0 µl for full length gels 16 20 cm to yield 100 or up to 500.